Extended Data Fig. 7: Trehalose functions in contributing to growth and infection of pathogens.

a, Disease symptoms of the etiolated soybean seedlings treated with 26 mM trehalose or 50 μM T6P, and then inoculated with P. sojae strain P6497-RFP. Disease symptoms were photographed at 2 dpi. b, Statistical analysis of the relative biomass of P. sojae (Right) and lesion size data (left). n = 10 (Lesion length) and n = 3 (Relative biomass) biologically independent seedlings for each treatment. c, Effect of trehalose concentration on lesion size and disease development in etiolated HC6 seedlings inoculated with psavh413 mutant. d, Statistical analysis of lesion length and relative biomass data quantified in c. n = 7 (Lesion length) and n = 3 (Relative biomass) biologically independent seedlings for each treatment. The relative biomass of P. sojae in soybean hypocotyls was determined by DNA-based qPCR. PsActin and GmCYP2 genes were used as internal controls. Experiments were repeated twice with similar results. Different letters indicate significantly different groups in b (P < 0.01, one-way ANOVA, Tukey HSD). Data of Relative biomass in b and d represent mean ± s.e.m. In b and d, boxplot centre lines show median value; upper and lower bounds show the 25th and 75th quantile, respectively; upper and lower whiskers show the largest and smallest values, respectively. The experiment was repeated twice with similar results.