Extended Data Fig. 9: Molecular characteristics of PsTREs of P. sojae.

a, The agarose gel shows a PCR result of PsTREs loci from individual P. sojae mutants. Mutants pstre1-M1, pstre1-M11, pstre2-M1, and pstre2-M18 are homozygous. WT represents wild-type P. sojae strain P6497. b, Growth of PsTRE1/2-knockout transformants. Photographs were taken at 3 and 7 days after Wild-type (WT) and PsTRE1/2-knockout transformants incubated on 10% vegetable juice (V8) medium at 25°C. c, Colony growth was calculated based on the colony diameter at 5 days. n = 4 biologically independent medium for each treatment. d, Knockout of PsTRE1/2 in P. sojae did not affect the ability of their infection in soybean hypocotyls. Photos were taken at 2 dpi. e, Statistics analysis of lesion length (Right) and relative biomass quantification (Left) of d. n = 3 (Relative biomass) and n = 7 (Lesion length) biologically independent seedlings for each treatment. Different letters indicate significantly different groups in b (P < 0.01, one-way ANOVA, Tukey HSD). Data in c and e (Relative biomass) represent mean ± s.e.m. Boxplot centre lines in e show median value; upper and lower bounds show the 25th and 75th quantile, respectively; upper and lower whiskers show the largest and smallest values, respectively. The experiment was repeated three times with similar results.