Extended Data Fig. 2: Overexpression of PsAvh413 affected plant development and defense response in Arabidopsis.
From: Phytophthora sojae boosts host trehalose accumulation to acquire carbon and initiate infection
a, Arabidopsis leaves expressing PsAvh413 increase the susceptibility to P. parasitica. Photos were taken at 2 dpi. b, c, Statistics analysis of relative biomass quantification (b) and lesion length (c) of a. PpUBC2 and AtActin genes were used as internal controls. n = 3 (b) and n = 15 (c) biologically independent leaves for each treatment. d, Western blot analysis of transgenic Arabidopsis plants carrying PsAvh413 using anti-HA antibody. Equal loading was confirmed by Coomassie brilliant blue (CBB) staining of total proteins. e, Representative images of wild-type plants and PsAvh413-transgenic Arabidopsis lines. PsAvh413 expression perturbed the growth and development of Arabidopsis plants. Four-week-old Arabidopsis seedling grown in soil were photographed (lower panel). Six-week-old Arabidopsis plants grown in soil were photographed (upper panel). Different letters indicate significantly different groups in b-c (P < 0.01, ANOVA, Tukey HSD). Data in b represent mean ± s.e.m. Boxplot centre lines in c show median value; upper and lower bounds show the 25th and 75th quantile, respectively; upper and lower whiskers show the largest and smallest values, respectively. The experiment was repeated three times with similar results.
