Extended Data Fig. 4: Membrane tension of the appressorium decreases during maturation.

Guy11 appressoria were stained with the rotor probe N+-BDP at 4.5 hpi (0 min) and FLIM images captured for 3 h and fluorescence lifetimes plotted.

Guy11 appressoria were stained with the rotor probe N+-BDP at 4.5 hpi (0 min) and FLIM images captured for 3 h and fluorescence lifetimes plotted.