Extended Data Fig. 6: Mapping spatial variation in chemical polarity of the plasma membrane in M. oryzae appressoria using the solvatochromic probe NR12S. | Nature Microbiology

Extended Data Fig. 6: Mapping spatial variation in chemical polarity of the plasma membrane in M. oryzae appressoria using the solvatochromic probe NR12S.

From: A molecular mechanosensor for real-time visualization of appressorium membrane tension in Magnaporthe oryzae

Extended Data Fig. 6

a, Chemical structure of NR12S. b,c Schematic illustrations showing the mechanism by which NR12S reports changes in chemical polarity and lipid phase. d-j, Intensity ratio chemical polarity maps in M. oryzae wild type strain Guy11 4 h appressoria. Images e, f, h and i are magnified areas of 4 h appressoria. The colour scale translates the intensity ratio values (n = 31 three independent repetitions of the experiment were performed). N+-BDP 4 h and N+-BDP 24 h FLIM images included for comparison. k-p, Intensity ratio chemical polarity maps of M. oryzae wild type strain Guy11 24 h appressoria. Images I, m, o and p are zoomed in areas of 24 h appressoria, Total observations n = 34 appressoria in 3 biological replicates. Images are representative of n = 3 independent repeats of the experiment. Images d, g, j, k and n, scale bars = 5 μm, images e, f, h, i, l, m, o and p scale bars= 1 μm. Image created with BioRender.com.

Back to article page