Fig. 2: Mechanosensor N+-BDP reveals variations in membrane tension of appressoria of the anthracnose pathogen C. higginsianum.
a, Time course of infection-related development of C. higginsianum development and maturation. Images show developing appressoria germinated on glass coverslips between 0 h and 24 h. Images are representative of n = 3 independent repeats of the experiment. Scale bars, 5 μm. b, Representative FLIM images of early and late N+-BDP rotor-stained appressoria. The colour corresponds to the fluorescence lifetime values expressed in nanoseconds, as shown in the key 2.5–4.5 ns. c, Dot plots showing the average fluorescence lifetime for early and late appressoria. Each dot corresponds to the average fluorescence lifetime obtained for an ROI drawn around the membrane of an individual appressorium in a 2D FLIM image. Total observations n = 40 appressoria examined in three biological replicates; typical range 20 appressoria; each biological replicate is colour coded (R1, replicate 1; R2, replicate 2; R3, replicate 3); data are presented as mean ± s.e.m. ****P < 0.0001, **P < 0.005 two-tailed unpaired Student’s t-test with Welch correction. Images are representative of n = 3 independent repeats of the experiment. Scale bars, 1 μm.
