Extended Data Fig. 3: Rotor dye N+-BDP calibration in M. oryzae appressoria incubated in glycerol.
Line graph showing the average fluorescence lifetime of N+-BDP stained appressoria of Guy11 incubated in different molar concentrations of glycerol. Values are means ± SEM for 3 biological replicates of the experiment, total observations n = 129 appressoria in 3 biological replicates; typical range 22–34 appressoria; data are presented as mean ± SEM. ***P < 0.0002 as determined by one-way analysis (ANOVA) with Dunnett’s multiple comparisons test.
