Extended Data Fig. 4: Active site mutants of G. vaginalis PulA.
From: Bacterial amylases enable glycogen degradation by the vaginal microbiome
a. 2 μg of active site mutants and wild type G. vaginalis PulA was loaded onto a Biorad (2-14% Bis-Tris) SDS page gel. This experiment was repeated twice demonstrating similar results (n = 2). b. Specific activity of active site mutants on glycogen. Inset graph shows specific activity data for G. vaginalis mutants. ∆AS1, ∆AS2, and ∆DBL represents D233A, D1317A, and both mutations respectively. Data is representative of three experimental replicates over two days. Error bars represent one standard deviation above and below the mean.
