Extended Data Fig. 7: Biofilm formation and motility of focal wrinkly host specialists and macrocolonies of Pl_MYb11, evolved isolates, ΔwspR mutants and PDE/DGC expressing derivatives.
From: Bacterial c-di-GMP has a key role in establishing host–microbe symbiosis
a, Biofilm formation of wspF, wspE, and rph mutants compared to Pl_MYb11 (ancestral median = dashed line) after two days shaking incubation microtiter plates. Illustration of biofilms with photographs of biofilms from test tubes for after 48h incubation. Scale bars = 4mm. b, Swarming motility and c, colony expansion of isolates wspF, wspE, and rph mutants were compared to Pl_MYb11 (ancestral median = dashed line) after 24 and 72h, respectively. b-c, Scale bars = 0.5 cm. a-c, Experiments were performed with min. 3 replicates/treatment and analyzed with ANOVA and fdr-corrected Dunnett post-hoc tests or a one-sided t-test. Box plots show median (center line), upper and lower quartiles (box limits) and the interquartile range (whiskers). d, Macrocolonies of ancestral Pl_MYb11 and three wrinkly isolates from the end of the evolution experiment (with single mutations in either wspF, wspE, or rph). e, Macrocolonies of ΔwspR mutants in Pl_MYb11 wspF, wspE, or rph background. f, Macrocolonies of wspF, wspE, or rph isolates expressing the phosphodiesterase PA2133 from plasmid, Pl_ MYb11 expressing the constitutively active diguanylate cyclase GCN4-WspR from plasmid, and empty vector controls. d-f, Macrocolonies were grown on tryptic soy agar supplemented with Congo Red for one (d,e) to three (f) days. Scale bars = 1mm.
