Fig. 3: Identification of GlpR as a membrane receptor for Glp.
a, Schematic representation of GlpR, with 3 transmembrane segments and the intrinsically disordered regions IDR1 (in red, residues 27–218, theoretical isoelectric points (pI) of 4.05) and IDR2 (in blue, residues 263–340, pI of 10.87). b, BLI sensorgrams of Glp binding to immobilized GlpR. Glp concentrations range from 100 nM (dark blue) to 1.56 nM (light green) in 2-fold dilutions. c, Cell fractionation and subcellular localization of Glp. Total (T), soluble (S) and membrane (M) fractions of Cglu or Cglu_Δglpr strains were obtained by differential centrifugation and analysed by western blot using an α-Glp antibody. d, Left: localization of mNeon-Glp in Cglu_Δglpr. Representative image in PC and mNeon-Glp fluorescent signal. Right: heat map representing the localization pattern of mNeon-Glp; 5,963 cells were analysed, from triplicate experiments. Scale bars, 5 μm.
