Extended Data Fig. 7: Flow cytometry gating and control in SYTOX Green and acriflavine assays.
The gating strategy and rationale in these assays are similar to the membrane potential assays. We were measuring and comparing the intracellular fluorescent dye concentration of the whole cell population, so we gated the tight single population, as shown in the three dot plots in the top panel. Both SYTOX Green and acriflavine can be excited by blue laser (488 nm) and emit green light (530/40 nm). The bottom left panel shows that the cells treated by SYTOX Green emitted stronger green light than the cells without treatment, and the same for acriflavine in the bottom right panel. This guaranteed that the fluorescent events obtained in Fig. 4 in the main text were from cells containing either SYTOX Green or acriflavine and the fluorescence shifts were positively correlative to the intracellular concentration difference of either of the fluorescent dyes. The dot plots and histograms were generated by BD InfluxTM Cell Sorter Sortware.
