Fig. 1: Chromatin interaction in T. cruzi genome compartments.

a, Normalized Hi-C interaction frequencies of a representative chromosome (chr9) shown as a two-dimensional heatmap at 10 kb resolution. CFDs identified using HiCExplorer and TADtools (triangles on the Hi-C map) are indicated in yellow. There is a correlation between the local minimum of the insulation score calculated using FAN-C, which represents the region between two self-interacting domains, and the CFDs identified with HiCExplorer and TADtools. The genomic positions of the core and disruptive genome compartments are represented by grey and yellow bars, respectively. ICE, iterative correction and eigenvector decomposition. b, Box plot of CFD length, depending on whether the domain is in the core or disruptive compartment. Centre lines of the box plots represent medians, box limits represent 25th and 75th percentiles, and whiskers span minimum-to-maximum values. CFD core median length = 230 kb (N = 25); CFD disruptive median length = 85 kb (N = 24). Significance was determined using an unpaired two-sample Wilcoxon test (two-sided). c, Mean interaction frequencies at all genomic distances at 5 kb resolution. For the analysis, chromosomes were classified into core (>80% core compartment), disruptive (>80% disruptive compartment) or mixed (Supplementary Table 2). d, Intra- and inter-chromosomal interactions between core and disruptive chromosomes.