Fig. 6: Self-interacting domain boundaries are conserved across cell types and strains.
From: Genome-wide chromatin interaction map for Trypanosoma cruzi
a–d, Results of the 3C assay. PCR amplification of cross-linked DNA template cut with EcoRI. PCR products were separated on 2% agarose gel stained with ethidium bromide. N, neighbour primers; D, distance primers. Two independent experiments were carried out. a, Region between position 363,000 and 427,000 of scaffold PRFA01000007. PCR products 300 nt and 166 nt in length were amplified with N and D primers, respectively. b, Region between position 272,663 and 513,267 of scaffold PRFA01000013. PCR products 275 nt and 171 nt in length were amplified with N and D primers, respectively. c, Normalized Hi-C maps of PFs and BFs at 10 kb resolution. CFD identified using TADtools are indicated with yellow triangles on Hi-C maps. The insulation score and local minima of the insulation score were calculated for both forms using FAN-C. Self-interacting chromosomal domains are conserved across African trypanosomes. d, We observed no differences (P > 0.05) in long-range (20 kb) versus short-range (<20 kb) contacts between PFs and BFs.
