Extended Data Fig. 8: Differences in 5mC methylation between co-existing genome in AMF heterokaryons.

(a) Violin-plots illustrating the relative density and differences in the degree of methylation among haplotypes. (b) Boxplots illustrating the difference in repeat 5mC methylation (left) and gene 5mC methylation (right) between A/B compartments in A4 and A5. The third quartile (edge of box), first quartile (edge of box), median (middle black line), outliers (dots) and range of data (whiskers) are shown. Two-tailed t test p-values for A4 MAT1 are 6e-13 and 0.0021 for repeats and genes, respectively. n = 8837 repeats and n = 10563 genes in A compartment, n = 13451 repeats and n = 9594 genes in B compartment. For A4 MAT2, p-values are 8.9e-07 and 2e-05 for repeats and genes, respectively. n = 8904 repeats and n = 10597 genes in A compartment, n = 13336 repeats and n = 9450 genes in B compartment. P-values for A5 MAT3 are 1.9e-10 and 0.35 for repeats and genes, respectively. n = 8251 repeats and n = 9350 genes in A compartment, n = 11367 repeats and n = 8009 genes in B compartment. For A5 MAT6, p-values are 1.1e-14 and 0.0002 for repeats and genes, respectively. n = 8389 repeats and n = 9447 genes in A compartment, n = 11676 repeats and n = 8112 genes in B compartment. (c) Boxplots illustrating the difference in methylation frequency between haplotypes in A4, A5 and G1(left), and the percentage of methylation per base within and among haplotypes in A4, A5 and G1(right).