Extended Data Fig. 1: Gut virome assembly is altered by micronutrient deficiencies in early life.
Weanling C57Bl/6 N male mice (n = 10) were given a control or multiple low-micronutrient diet (deficient in vitamin A, B12, B9 [folate], zinc, and iron) ad libitum for 28 days. Shotgun metagenomics sequencing was performed on mouse fecal samples collected at Day 0 and Day 28. (a) Shannon alpha diversity of virome at each time point Day 0 (P = 0.28) and Day 28 (Wilcoxon, P = 0.92) (b) Virile bar plot between groups at the genus level on Day 0 and 28. (c) Virus/bacteriophage altered at the genus level following dietary treatment. All P values were FDR-adjusted (Q). *Q < 0.05, **Q < 0.01, ***Q < 0.001, ****Q < 0.0001. Data shown are from 10 individual mice per group.
