Extended Data Fig. 8: Representative amplification plots of the 16S and sip PCR-qPCR assays in a placental DNA sample. | Nature Microbiology

Extended Data Fig. 8: Representative amplification plots of the 16S and sip PCR-qPCR assays in a placental DNA sample.

From: Placental Streptococcus agalactiae DNA is associated with neonatal unit admission and foetal pro-inflammatory cytokines in term infants

Extended Data Fig. 8

Amplification plots were obtained using the multiplex qPCR assay targeting the sip (green) and 16S (red) GBS genes and the human RNaseP (RPPH1, blue) gene. The relative abundance of the target is expressed using the Ct (cycle threshold) value, which is inversely associated with the signal. Rn (normalized reporter value) represents the fluorescence of the reporter dye normalized to the signal of the passive reference dye for a given reaction. The ΔRn is the Rn value of an experimental reaction minus the Rn value of the baseline signal generated by the instrument. This parameter indicates the magnitude of the fluorescent signal generated in the qPCR assay.

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