Extended Data Fig. 7: Comparison of sip and 16S PCR-qPCR signals for GBS in placental samples. | Nature Microbiology

Extended Data Fig. 7: Comparison of sip and 16S PCR-qPCR signals for GBS in placental samples.

From: Placental Streptococcus agalactiae DNA is associated with neonatal unit admission and foetal pro-inflammatory cytokines in term infants

Extended Data Fig. 7

Correlation between the sip and 16S Ct values obtained in the nested PCR-qPCR assays targeting the two GBS genes (n = 44) using the data from the validation study. Samples with undetectable sip signal are plotted with Ct = nd (not detected, n = 8). Where samples were positive using both methods, the Ct count was higher for sip than for the 16S rRNA PCR-qPCR (mean difference = 2.4, 95% CI = 1.0 to 3.7, P = 0.0009). Pearson’s correlation coefficients (r) and two-tailed p values are reported for 16S Ct <30 (p < 0.0001) and Ct >30 (p = 0.56). Ct denotes cycle threshold.

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