Extended Data Fig. 5: Tyr119 in PPIase is essential for interacting with BfUbb. | Nature Microbiology

Extended Data Fig. 5: Tyr119 in PPIase is essential for interacting with BfUbb.

From: Bacteroides fragilis ubiquitin homologue drives intraspecies bacterial competition in the gut microbiome

Extended Data Fig. 5: Tyr119 in PPIase is essential for interacting with BfUbb.

a, Confocal microscopy views of B. fragilis GS077S, GS077-PPIGS084(110-120), GS084I, and GS084-PPIGS077(110-120) treated with BfUbb after 5 hours. In strain GS077-PPIGS084(110-120), amino acids 110-120 of PPIaseGS077 were replaced with the corresponding amino acids 110-120 of PPIaseGS084. In strain GS084-PPIGS077(110-120), amino acids 110-120 of PPIaseGS084 were replaced with the corresponding amino acids 110-120 of PPIaseGS077. b, Confocal microscopy views of B. fragilis GS077S, GS077-PPIE118A/Y119E, GS084I, and GS084-PPIA118E/E119Y treated with BfUbb for 5 hours. c, Confocal microscopy views of B. fragilis GS077S, GS077-PPIE118A, GS077-PPIY119E, GS084I, GS084-PPIA118E, and GS084-PPIE119Y treated with BfUbb for 5 hours. For a, b, and c, cellular membranes are stained with lipophilic dye FM 1-43 (green); scale bars, 5 μm; the images represent at least 20 images from three independent experiments.

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