Fig. 1: Ipflufenoquin acts against DHODH. | Nature Microbiology

Fig. 1: Ipflufenoquin acts against DHODH.

From: Aspergillus fumigatus strains that evolve resistance to the agrochemical fungicide ipflufenoquin in vitro are also resistant to olorofim

Fig. 1: Ipflufenoquin acts against DHODH.The alternative text for this image may have been generated using AI.

a, MIC determination of ipflufenoquin (0.175–50 mg l−1) against A. fumigatus MFIG001 in RPMI-1640 (black) using the EUCAST methodology and with addition of 10 mM uridine and uracil (grey). OD600 was measured after 48 h. Three biological replicates were assessed. Data are presented as mean ± s.e.m. b, Molecular docking of FMN and orotate (left in cyan and blue, respectively) and olorofim and ipflufenoquin (right, orange and purple, respectively) to an Alphafold2 model of A. fumigatus DHODH. The pocket of the active site is shown in grey. c, Protein inhibition assay of ipflufenoquin towards wildtype and G119 variants of DHODH. At least five biological replicates were assessed. Data are presented as mean ± s.e.m. (n = 5). d, MIC determination of G119 variants (G119C, G119S and G119A) against ipflufenoquin (right) and olorofim (left) according to EUCAST methodology. OD600 was measured after 48 h. Three biological replicates were assessed. Data are presented as mean ± s.e.m.

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