Extended Data Fig. 10: Epifadin leads to depolarization of the bacterial membrane and rapid cell lysis of S. aureus. | Nature Microbiology

Extended Data Fig. 10: Epifadin leads to depolarization of the bacterial membrane and rapid cell lysis of S. aureus.

From: Commensal production of a broad-spectrum and short-lived antimicrobial peptide polyene eliminates nasal Staphylococcus aureus

Extended Data Fig. 10

(a) S. aureus USA300 JE2 cells were applied to an agarose pad, onto which 2 µL of extracts (50 mg/mL) of the epifadin producer IVK83 (left) or ΔefiTP (right) had been previously spotted. Image acquisition was started in the surrounding of the respective extract spot 15 min after S. aureus application. The agarose contained FM4-64 (red, 0.25 µg/mL, membrane dye) and Sytox Green (green, 0.25 µM, only visible upon membrane barrier malfunction). Representative micrographs are depicted, all adjusted in the Sytox green channel to the same settings for qualitative comparison. White scale bar, 5µm. (b) Time-resolved effects of extracts of IVK83 wild type and ΔefiTP on the membrane potential of S. aureus NCTC8325 as monitored by DiOC2(3) staining. CCCP (5 µM), positive control; DMSO, untreated control. Mean and s.d. of two biological with two technical replicates (n = 4). Black arrow, time point of compound addition.

Source data

Back to article page