Fig. 3: Detection, isolation and absorption spectrum of epifadin. | Nature Microbiology

Fig. 3: Detection, isolation and absorption spectrum of epifadin.

From: Commensal production of a broad-spectrum and short-lived antimicrobial peptide polyene eliminates nasal Staphylococcus aureus

Fig. 3

Chromatogram and absorption spectrum of epifadin on RP-HPLC column. a,b, HPLC-UV chromatograms (λ = 383 nm) of DMSO–PA extract of IVK83 wild type (a) and ΔefiTP mutant (b). A prominent peak (black arrow) of epifadin is only present in the wild-type sample with a retention time of ~15 min at 383 nm. c, UV chromatogram (383 nm) of the epifadin peak is shown. UV spectra of the active compound at 15.2 min and 15.6 min from 190 nm to 450 nm are shown (methanol as a solvent causes a strong absorbance at 200 nm). The absorption maxima of the epifadin peak are indicated (peptide bond, 210–230 nm; phenylalanine, 280 nm; and polyene moiety, 330–410 nm).

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