Extended Data Fig. 8: Analysis of the attG integration site motifs.
From: Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites
a, Consensus sequence of the six highest hotspot integration sites. A total of 20 bases around the cleavage point was inputted into the WebLogo program (http://weblogo.berkeley.edu/) to generate the motif. The cleavage occurs between the -1 and 1 bases. Bits refers to the information content. b, Recombination frequencies of attCaadA7 donor sites into the mutated ybhO hotspot and attI1 sites. The 4 nucleotide sequences indicated in the axis-x legends correspond to the position 6 to 9 given that position -1 corresponds to the G of the cleaved triplet site. The wt ybhO and attI1 sites are indicated in red at the top of the bars. Bar charts show the mean of three independent experiments (n = 3, individual plots) except for ybhO wt CAGC (with IntI1) for which n = 8. Error bars show the standard deviation. Statistical comparisons (Student’s t test) are as follow: ns. (not significant); all two-sided. Grey asterisk (*) indicates the recombination frequency was below detection level.
