Extended Data Fig. 3: Cassette integration events in genome in presence of an integron.
From: Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites
a, Experimental setup of the cassette integration assay (as in Fig. 3a). b, Electrophoresis migration of PCR products. Several PCR products are sequenced and the integration sites are indicated (att sites and gene location). c, Recombination frequencies of attCaadA7 donor sites into the att sites and genome. Integration sites are indicated in the axis-x legends. Bar charts show the mean of four independent experiments (n = 4, individual plots) and error bars show the standard deviation. Statistical comparisons (Student’s t test) are as follow: na. (not applicable); Grey asterisk (*) indicates the recombination frequency was below detection level. ts: thermosensitive; MW: Molecular Weight Marker, kb: kilobases.
