Fig. 5: phi3T_93 promotes lysogeny.
From: Antagonistic interactions between phage and host factors control arbitrium lysis–lysogeny decision
a, Strains lysogenic for phi3T wt and evolved ΔaimR phages were MC induced. The number of lysogens were quantified using 168 Δ6 amyE::PSpank-empty or 168 Δ6 amyE::PSpank-phi3T_93 (wt/L23D) as the recipient strains. b–d, B. subtilis strains either devoid of (purple) or expressing wt phi3T_93 (red) or phi3T_93L23D (grey) were infected with phage phi3T wt at an MOI of 1:10 (phage:bacteria). For all infection experiments, growth (OD600) readings (b), phage titres (c) and the relative number of lysogens (d) were determined after 3 h. Results are presented as p.f.u.s ml−1 in c, or c.f.u.s ml−1 normalized by p.f.u.s ml−1 and presented as the c.f.u. of an average phage titre (1 × 109 p.f.u. ml−1) in a and d. In a, c and d, geometric mean and geometric s.d. are shown (n = 3), and in b, mean ± s.d. are shown (n = 3). An ordinary one-way ANOVA of log10-transformed data, followed by Dunnett’s multiple comparisons test, was performed to compare mean differences in titre or relative lysogen numbers. Comparisons were performed against results for the same phage (wt phi3T or evolved) infecting 168 Δ6 or 168 Δ6 amyE::PSpank-empty strain. Adjusted P values: ****P < 0.0001; *P < 0.05.
