Extended Data Fig. 1: Characterisation of phi3T master repressor.
From: Antagonistic interactions between phage and host factors control arbitrium lysis–lysogeny decision
(a-b) Lysates containing phages phi3T wt or phi3T Δphi3T_97 were used to infect strains 168 Δ6 or 168 Δ6 amyE::PSpank-phi3T_97, which overexpresses phi3T_97. The number of resulting plaques and lysogens were then quantified. The geometric mean and SD are presented (n = 3). (c) B. subtilis strains either devoid of (black) or expressing phi3T_97 (pink) were infected with phage phi3T wt at an MOI of 1:10 (phage:bacteria). The mean and SD of OD600 readings (n = 3) are presented. (d-e) After 3 h, the number of resulting plaques and relative number of lysogens were quantified. The geometric mean and SD are presented (n = 3). The results are shown as PFUs ml-1 or as CFUs ml−1 normalised by PFUs ml-1 and represented as the CFU of an average phage titer (1×109 PFU ml-1). Values below detection limit (1 ×101 PFUs ml−1 or CFUs ml−1) are not marked. In panel (d) and (e) a two-tailed t-test was performed to compare mean differences; p values indicated were as follows: ****p < 0.0001.
