Extended Data Fig. 4: AimR is an antiterminator in phage SPβ.
From: Antagonistic interactions between phage and host factors control arbitrium lysis–lysogeny decision
(a) Schematic view of the regions of SPβ DNA cloned into the pDG1663+ PSpank reporter plasmid. TT*: mutated transcriptional terminator. (b) B. subtilis strains carrying pDR110 (empty or AimRSPβ) and the reporter plasmids were grown to OD600 0.2. IPTG was added for induction of the PSpank promoter in the pDR110 plasmid (empty or AimR). β-gal expression was measured using the Miller method. Data presents the mean and SD (n = 3). Two-tailed t-tests were performed to compare mean differences; p values indicated were as follows: ***p = 0.0004; ns (not significant). replicates. (c) Predicted minimum free energy secondary structures of the terminator for the wt and TT* (evolved) sequence according to RNAfold. Colours are representative of positional entropy in the structure. Black arrow indicates the mutated bp.
