Fig. 1: Microbiota shift and plant disease driven by Xanthomonas Leaf131 in rbohD knockout plants.
a, Composition of synthetic bacterial communities SynCom-137 + Xanthomonas Leaf131 or SynCom-137 in rbohD or rbohD/RBOHD plants was compared with Col-0 wild-type plants. Effect size represents percentage of total variance explained by genotype (shown by dot size and absolute value) and statistical significance is expressed with P values determined by PERMANOVA (Benjamini–Hochberg adjusted, n = 16). Number of differentially abundant strains (as shown in b) is shown by dot colour. b, Heatmap shows subset of strains in SynCom-137 with significant log2 fold changes (log2FC, P < 0.05) in rbohD or rbohD/RBOHD compared with Col-0 wild-type plants in the presence (+) or absence (−) of Xanthomonas Leaf131. Black rectangles show significant changes, P < 0.05 (n = 16, two-sided Wald test, Benjamini–Hochberg adjusted). Complete heatmap of all strains in SynCom-137 is shown in Extended Data Fig. 1c. c, Fresh weight of aboveground plant tissue of Col-0, rbohD and rbohD/RBOHD mock inoculated, with SynCom-137 or SynCom-137 + Xanthomonas Leaf131. Box plots show the median with upper and lower quartiles and whiskers present 1.5× interquartile range (n = 16, two-sided Mann–Whitney U test, P values indicated above box plots). Corresponding plant phenotypes are shown in Extended Data Fig. 1d. d, CFU counts of Pseudomonas Leaf434 per gram plant fresh weight after inoculation of germ-free Col-0, rbohD and rbohD/RBOHD plants with Pseudomonas Leaf434 as single inoculation or in binary inoculation with Xanthomonas Leaf131 Tn7::Gm-lux. Box plots show the median with upper and lower quartiles and whiskers present 1.5× interquartile range (n = 12, two-sided Mann–Whitney U test, P values indicated above box plots).
