Fig. 4: T2SS Xps requirement for full virulence and fitness of Xanthomonas Leaf131 in planta.

a, Phenotype of 5-week-old Col-0 plants (blue arrow) and rbohD plants (green arrow) mock inoculated or with Xanthomonas Leaf131 wild type (WT) or T2SS mutants xps, xcs and xpsxcs. Scale bars, 1 cm. b, Measurement of fresh weight from plants shown in a. c, CFU counts of Xanthomonas Leaf131 per gram plant fresh weight from samples in b. Box plots show the median with upper and lower quartiles and whiskers present 1.5× interquartile range. Significant differences in b (n = 20) and c (n = 12) were calculated with ANOVA and two-sided Tukey’s honest significant difference post hoc test (letters indicate significance groups, α = 0.05). Log reduction of bacterial abundance shown in Source Data Fig. 4.