Fig. 5: Additional virulence factors contribute to leaf degradation and virulence of Xanthomonas Leaf131.
a, Leaf discs of 5-week-old rbohD plants were mock (10 mM MgCl2) inoculated or inoculated with Xanthomonas Leaf131 wild type (WT) or gene deletion mutants (OD of 0.02) and incubated for 24 h. b, Fresh weight of aboveground plant tissue of 5-week-old gnotobiotic rbohD plants, either mock inoculated or inoculated with Xanthomonas Leaf131 wild type or gene deletion mutants. c, Leaf discs of 5-week-old rbohD plants were mock treated (0.5× LB) or treated with cell-free supernatant (0.22 µm filter sterilized) of liquid cultures from Xanthomonas Leaf131 wild type and gene deletion mutants. Leaf discs were incubated for 24 h at 22 °C. Black circles, rectangles and squares indicate data from three bacterial cultures. d, CFU counts of Xanthomonas Leaf131 per gram plant fresh weight from samples in b. Box plots show the median with upper and lower quartiles and whiskers present 1.5× interquartile range. Significant difference in a (n = 8), b (n = 20), c (n = 24) and d (n = 12) of gene deletion mutants compared with Leaf131 wild type was determined by two-sided Mann–Whitney U test, and P values are indicated above box plots.
