Extended Data Fig. 2: Xanthomonas disrupts leaf tissue cohesion and requires plant wound. | Nature Microbiology

Extended Data Fig. 2: Xanthomonas disrupts leaf tissue cohesion and requires plant wound.

From: Leaf microbiome dysbiosis triggered by T2SS-dependent enzyme secretion from opportunistic Xanthomonas pathogens

Extended Data Fig. 2

a) Time-course of leaf discs from five-week-old rbohD plants inoculated with Xanthomonas Leaf131 (OD=0.02). Scale bar represents 1 mm. b) Leaf discs of rbohD plants in 96-well plate after 48 hours incubation with Xanthomonas Leaf131 were vortexed for two seconds. Scale bar represents 1 mm in left and middle panel, and 0.5 mm in right panel. c) Leaf discs of five-week-old Col-0 plants after 48 hours incubation with Xanthomonas Leaf131. Scale bar represents 1 mm in left and middle panel, and 0.5 mm in right panel. Experiment shown in panel A-C was repeated at least ten times. d) Leaves of five-week-old rbohD plants floating in water with undamaged leaf edge (left panels) or wounded leaf edge (right panels) were mock inoculated (10 mM MgCl2) or with Xanthomonas Leaf131 or Leaf148 (OD=0.02).

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