Extended Data Fig. 1: Schematic representation of the screen workflow and data processing.
a) Schematic screen workflow. Details described in Methods. b) Schematic of data processing. Details described in Methods. c) Pearson replicate correlation between the triplicates of each strain. d) Scatterplot for comparison of mean residuals of wt vs. wt (y-axis) and CBASS vs. wt (x-axis). Each point represents the mean residuals (of three replicates) of the lines of best fit for a given condition (compound, concentration. There are very few conditions for which wt vs. wt residuals (measure of noise) surpasses the stringent cut-off = 0.2. All conditions for which the CBASS vs. wt mean of residuals is higher than 0.2 are marked in blue. SMX = Sulfamethoxazole, TMP = Trimethoprim, Mero = Meropenem, PenG = Penicillin G, Amox = Amoxicillin. Note that only TMP, SMX and PenG were considered as truly CBASS dependent, as Amox and Mero were noisy and did not pass the additional cut-off of score vs wt-wt residuals of a two-sided t-test Benjamini-Hochberg adjusted p-val<0.05. n equals the number of independent biological replicates.
