Extended Data Fig. 2: Impact of CBASS in antifolates treatment of V. cholerae.
a) Pearson correlation coefficient of four MIC curves per strain and drug (shown in Fig. 2b) obtained from growth experiments in 96 well plates. b) Pearson replicate correlation based on AUC10h for checkerboard assays. The experiments were performed in quadruplicates, thus all possible 6 unique pairwise correlations are shown (a & b). c) MIC curves acquired in identical conditions to checkerboard experiments (384 well plates, N = 16) for TMP (left) and SMX (right). Drug concentrations and fitness are represented in the x-axis and y-axis, respectively. Fitness was calculated as the ratio between AUC10h with and without added drug. d) Simplified visualization of synergy between antifolates. MIC curves for wild-type (right) and ΔCBASS (left) showing that low TMP concentration decreases SMX MIC (triangles) in comparison to SMX alone (circles) are shown. e) Estimation of minFICi for isobole 0.1: FICis are calculated along drug concentrations normalized by their respective MICs, to determine the minimal minFICi0.1. n equals the number of independent biological replicates (c, d & e).
