Extended Data Fig. 7: PRO-seq reveals aborted transcription at invertons and prophages.
From: Precision run-on sequencing (PRO-seq) for microbiome transcriptomics
(a) Stranded coverage data across four invertons from US2 and US3 is shown, with inverted repeats marked with blue triangles. Coordinates and directionality of coincident genes are given below the coverage plots. Decomposition of PRO-seq reads into 3’ and 5’ ends shows that transcription is initiated within the inverton and terminated just downstream. (b) Four examples of transcription across prophages, highlighting the complementary nature of PRO-seq and RNAseq data for observing the transcription of mobile genetic elements. The bounds of CI-like transcriptional regulators are demarcated by yellow arrows. Teal arrows give the bounds of genes encoding proteins of unknown function.
