Extended Data Fig. 5: Electron acceptors not observed to stimulate growth.
From: Dietary- and host-derived metabolites are used by diverse gut bacteria for anaerobic respiration
(a) GC-MS analysis of resveratrol-spiked media before and after E. lenta DSM2243 growth and LC-MS analysis of resveratrol-spiked media before and after H. filiformis DFI.9.20 growth. The low solubility of resveratrol hindered experiments to assess whether this electron acceptor supported respiratory growth. (b) Potential modification flow for (+)-catechin and (−)-epicatechin, and GC-MS of media spiked with either (+)-catechin or (−)-epicatechin before and after E. lenta DSM2243 or S. wadsworthensis DFI.4.78 growth. (c) Resting cell suspensions of E. lenta and S. wadsworthensis in buffer supplemented with 1mM formate assayed cellular ATP concentrations after incubation with buffer alone or (−)-epicatechin, data are mean ±SD (n = 3 technical replicates). Support for identification of catechin and epicatechin derivatives in (B) is provided in Supplementary Figs. 2 and 3.
