Fig. 1: Strain overview.
From: OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression
a, Schematic time line of the history of the Komagataella species as well as the available type-strain and patent-strain deposits. SIBIA, Salk Institute Biotechnology/Industrial Associates. b, Phylogenetic tree of the K. phaffii strains with node lengths shown. The tree was constructed using a maximum likelihood method and the Hasegawa–Kishino–Yano model. Node lengths of <0.0001 were neglected. c, Summary of the coding mutations in the Phillips Petroleum Company strain and type strains compared with the CBS 7435 reference genome. The specific mutations are described in the second column, with the CBS 7435 amino acid as the reference amino acid (although the original genetic makeup is at present in the type strains and it is the CBS 7435/NRRL Y-11430 that mutated). The mutations indicated with asterisks were also reported by Brady and colleagues14. All mutations found in these strains are concentrated in six locations. The mutations in SEF1, ROP100/RSF2 and HOC1 are shared by all type strains. The other mutations (in SRB7, RAD18 and PRP46) are present in one of the type strains (NCYC 2543, CBS 2612 and NRRL Y-7556, respectively).
