Extended Data Fig. 5: Surface display phenotype of Pichia pastoris strains NRRL Y-11430, NCYC 2543 and OPENPichia.
From: OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression
Human lysozyme was fused to the C-terminal part of Sag1p (which contains a GPI anchor) and with an N-terminal FLAG tag and a C-terminal V5 tag for detection in flow cytometry. The resulting fusion protein was expressed using the AOX1 promoter. Copy number was determined and two clones with a copy number of 1 were selected for each strain. Cells were plotted by a 5% quantile contour plot with outliers presented as dots. Quadrant gates were set using non-stained and single-stained controls. The number of technical replicates is 3. The gating strategy is shown in Extended Data Fig. 6.
