Extended Data Fig. 6: Gating strategy of the yeast surface display experiment.
From: OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression
At least 10,000 events are captured on a FACSMelody instrument and data is analysed with the FlowJo software. First, debris is gated out, then single cells are gated using both the side scatter (SSC) and the forward scatter (FSC), and finally living cells are gated by using the Live/Death stain eFluor506. The resulting living single Pichia cells are analysed based on FLAG and V5 signal. The gates of IV and V were determined based on non-stained and single-stained controls.
