Fig. 3: Effect of the HOC1 truncations on plasmid transformation efficiency and HOC1 mRNA abundance in the resulting strains.
From: OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression
a, Transformation efficiency of a PAOX1- (top) and a PGAP-based plasmid (bottom) in the two wild-type and two HOC1-engineered strains. The analysis was performed as described in Methods. Technical repeats (1 ≤ n ≤ 10) are shown as semi-transparent data points; the solid data points and error bars are the group means and the 95% confidence intervals (where available), respectively, as estimated by the linear model. b, Levels of HOC1 mRNA, as determined using RT–qPCR, in the different strains. Individual biological repeats (n = 2 or 3) and their mean are shown as points and bars. Each biological replicate was determined from three technical replicates. c, Schematic representation of the primers used in the RT–qPCR experiment: primer pair 1 binds near the start codon of HOC1, whereas primer pair 2 binds close to the premature stop codon. The asterisk indicates the position of the premature stop codon in NRRL Y-11430 and the HOC1-truncated mutants of NCYC 2543. The stop codon is absent in the NCYC 2543 strain.
