Extended Data Fig. 1: Expression comparison between NCYC 2543 and NRRL Y-11430.
From: OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression
The proteins were expressed using the GAP or AOX1 promoter (three biological replicates per strain, promoter and protein). As controls, both wild type strains were grown and analysed as well. Supernatant samples were treated with EndoH to remove N-glycans and samples were analysed on SDS–PAGE. EndoH is also visible on the gels at around 30 kDa. The arrows indicate the expected location of the expressed protein band, based on the theoretical size.
