Extended Data Fig. 3: Overview of the HOC1 genome engineering strategy.
From: OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression
a. Alignment of a part of the HOC1 gene as present in NRRL Y-11430 vs. NCYC 2543, showing the frameshift resulting in a premature stop codon in the NRRL Y-11430. b. Resulting genomic HOC1 sequence upon split-marker-based gene editing. Two strategies were followed where either the single base pair deletion (indicated with *) resulting in the Hoc1p truncation and a Lox72 scar is introduced downstream of the stop codon; or where an additional 115 bp deletion downstream of the resulting stop codon and Lox72 scar is introduced.
