Extended Data Fig. 10: Comparison of MraY(WT) and MraY(T23P) structures in the YES complex.

(a) Overlay of densities of MraY(WT) (EMD-29641) (green) and MraY(T23P) (purple) viewed in the plane of the membrane. (b) Enlarged view of the densities around the T23P mutant. Residues are shown in stick representation. Residues 21-23 are labeled for reference. (c) As in B for the wild-type complex. (d) Hydrogen bonding network observed in MraY(T23P) (left, purple) compared to WT (right, green) at the mutagenesis site involving Y21, Y227, and K358. (e) Similar to D, overlay of the two models highlighting the conformational differences of residue Y21.