Fig. 3: Amino acid substitutions in hyperactive MraY variants localize to the extracytoplasmic surface of the dimer interface. | Nature Microbiology

Fig. 3: Amino acid substitutions in hyperactive MraY variants localize to the extracytoplasmic surface of the dimer interface.

From: Synthesis of lipid-linked precursors of the bacterial cell wall is governed by a feedback control mechanism in Pseudomonas aeruginosa

Fig. 3

a, Tenfold serial dilutions of P. aeruginosa ΔponB ΔlpoA cells harbouring expression plasmids producing the indicated MraY variant were plated on VBMM with or without IPTG to induce the MraY variants as indicated. b, Structural model of P. aeruginosa MraY created using AlphaFold24 in cartoon viewed from the plane of the membrane. Residues altered in hyperactive variants tested in a are shown in stick representation (purple), while those residues previously implicated in catalysis are shown in red. c, Structural model of the MraY dimer created using AlphaFold24. Surface representation of one protomer is shown in grey, with the residues altered in hyperactive variants coloured in purple. The other protomer is shown in cartoon representation (green) for simplicity. Left: periplasmic view of the dimer. Right: view from the plane of the membrane.

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