Extended Data Fig. 9: Crossmatch reactivity of group O plasmas towards enzymatically converted A1 RBCs.
The crossmatches were performed before and after treatment with AmGH36A (1 μM) or a one-pot treatment with AmGH36A (1 μM), AmGH95B (0.02 μM) and AmGH35A (0.2 μM). The colour-scale (red-pink-white) corresponds to strength of crossmatch reactivity towards group O plasma samples (n = 100, a-j, 1-10), where red is the strongest reaction (4 + ) and the shades of pink represent gradually weaker reactions (3 + , 2 + , 1 + ). Minus represents a negative crossmatch. A minus in brackets shows a reaction between negative and very weakly positive but not fulfilling the criteria for a positive (1 + ) reaction. Untreated RBCs of secretor/non-secretor background were crossmatched with only a subset of O plasmas (n = 10, 1a-1j) to get a baseline level of reactivity for comparison with enzyme-treated cells. The figure allows tracking changes in crossmatch reactivity of individual plasmas with the single and one-pot enzyme treatments.
