Extended Data Fig. 9: Regulation of cGAS activity by E2-mediated poly-cGASylation.
a, HPLC analysis and quantification of the 3′,2′-cGAMP synthesized by cGAS and variants as shown in Fig. 5a, with concentrations of ATP, GPT, and Mn being 250 uM, 250 uM, and 2.5 mM, respectively. b, HPLC analysis and quantification of the 3′,2′-cGAMP synthesized by cGAS and variants as shown in Fig. 5b, with the concentration of ATP, GTP, or Mn2+ in the reaction reduced by half compared to those in a. All data are representative of three independent experiments.
