Extended Data Fig. 4: R. buchneri infection in response to bioenergetic alterations.
From: Bacterial reprogramming of tick metabolism impacts vector fitness and susceptibility to infection
(a) Schematic of microbial infection upon glycolysis or OxPhos inhibitor treatment. 1 × 106 ISE6 cells were treated with 50 mM 2-deoxy D-glucose (2-DG), 0.1 µM rotenone (Rot), 0.5 µM antimycin (Anti), 0.5 µM oligomycin (Oligo) or 20 µM 2,4-dinitrophenol (2,4-DNP) for 1 hour prior to infection with R. buchneri at MOI 50. Cells were collected 2 days post-infection for bacterial quantification via RT-qPCR. Infected cells without inhibitor treatment (-) served as controls. (b) R. buchneri infection was measured using the citrate synthase (gltA) gene by RT-qPCR (n = 6 for each condition, n represents individual culture wells). Data are representative of two independent experiments with mean ± SEM. Statistical significance was evaluated by Kruskal Wallis test followed by Dunn’s multiple comparisons test. Significant p values (<0.05) are displayed in the figure.
