Extended Data Fig. 6: Effect of abat silencing on tick fitness.
From: Bacterial reprogramming of tick metabolism impacts vector fitness and susceptibility to infection
(a-d) I. scapularis nymphs were injected with siRNA (blue) or scrambled control (grey) for 4-aminobutyrate aminotransferase (abat) and allowed to feed on uninfected C57BL/6 mice for 3 days. Fitness parameters were recorded. (a) Silencing efficiency (n = 11 and 10); (b) tick attachment (n = 76 and 81); (c) weight (n = 37 and 34); and (d) survival (n = 30 and 31). (a-d) Data are representative of two independent experiments with mean ± SEM where n = an individual tick. Statistical significance was evaluated by (a, c) two-sided t-test with Welch’s correction for unequal variances (b) Fisher’s exact test (d) Log-rank (Mantel-Cox). NS = not significant. Significant p values (<0.05) are displayed in the figure.
