Extended Data Fig. 2: SLC35A2 KO differentially impacts CXCR4-tropic and CCR5-tropic HIV-1, related to Figure 2e.
From: Host cell glycosylation selects for infection with CCR5- versus CXCR4-tropic HIV-1
a. Percentage of CD4+ T cells staining positive for HIV-Gag, relative (rel) to CD19 KO, at 6 dpi (MOI=0.02, same infections as depicted in Fig. 2e, different measurement of HIV-1 infection). b. RT activity over time from spreading infections (MOI=0.02) in primary CD4+ T cells from two donors to determine coreceptor tropism. Results are separated by expected coreceptor tropism. Donor numbering is consistent within this figure and with Fig. 2e. Additional information on the strains used for infections is presented in Extended Data Table 1.
