Fig. 3: Impact of altering insertion sequence, deletion length and AA residue substitution on EV-A71 growth. | Nature Microbiology

Fig. 3: Impact of altering insertion sequence, deletion length and AA residue substitution on EV-A71 growth.

From: Deep mutation, insertion and deletion scanning across the Enterovirus A proteome reveals constraints shaping viral evolution

Fig. 3: Impact of altering insertion sequence, deletion length and AA residue substitution on EV-A71 growth.

a, Area plot showing the proportion of variants within Enrich2 score bins for all variable inserted residues. The two-sided chi-squared statistics are χ² = 108.69 and d.f. = 38. b, Heat maps showing the Enrich2 scores in VP3 N termini VP1, 2A(pro) and 3A. The asterisk (*) represents the catalytic residue in the 2A(pro), H21. Black squares represent variants that were not analysed due to low variant counts in the input library. The diagram on the left explains the y axes of the maps. c, Area plot showing the proportion of variants within Enrich2 score bins for different deletion lengths. The two-sided chi-squared statistics are χ² = 63.252 and d.f. = 4. d, Bar plots showing Enrich2 scores for different lengths at the N termini of VP1, 2A(pro) and 3A. Alternating shaded regions highlight the boundaries between viral proteins. e, Area plot showing the proportion of variants within Enrich2 score bins for all different AA substitutions. The two-sided chi-squared statistics are χ² = 991.35 and d.f. = 38.

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