Fig. 6: InDels as a contributor of EV-A species evolution.
a,e, Heat map plotting the Enrich2 scores for mutations at the InDel hotspot regions present at the N (a) and C (e) termini of VP1. Phylogenetic tree based on the full coding sequences of EV-A species (n = 107 sequences). b,f, The phylogenetic tree is coloured by the gap lengths observed at the N (b) and C (f) termini of VP1. c,g, Multiple sequence alignments of the N (c) and C (g) termini of VP1. d,h, Structural alignment of EV-A71 (PDB:8E2X) and CV-A6 (PDB:7QW9) pentamer at the N (d) and C (h) termini. Mean relative enrichment scores for AA changes were mapped onto the EV-A71 and CV-A6 structures in h. The asterisk (*) in the heat map represents the residue H21 present in the catalytic triad of the 2A(pro). The cleavage sites where 3CD cleaves VP3 and VP1 and the 2A(pro) cleaves the capsid proteins from the replication proteins are shown. Black squares for AA changes are the WT sequence. Black squares for deletions are variants removed due to low variant counts in the input library. L is equal to the length of the different gaps. Del., deletion; ins., insertion.
