Extended Data Fig. 4: Intestinal Inflammation.
From: Bacterial and host fucosylation maintain IgA homeostasis to limit intestinal inflammation in mice
Loss of Bacterial Fucosylation Promotes Intestinal Inflammation and Increases Colitis Risk. (a) Representative plots of flow cytometry and the percentage of AAL positive gut bacteria in GF mice transplanted with fecal microbiota of WT mice (FMT-WT) and Fut2-/- mice (FMT-Fut2-/-). (b-f) Female, 6-week-old, antibiotic-pretreated C57BL/6 J mice were colonized with BFWT9343 or BFΔΔ9343 strains for 2 weeks. BFWT9343 (blue) and BFΔΔ9343 (pink) colony-forming units (CFU) in fecal pellets (b) were determined daily for 6 days. Body weight changes (c), representative images of the cecum (d), and representative H&E staining of the cecum (e) and colon (f). Scale bar: 100 µm. (g) TIMER2.0 analysis of correlation between fut2 and B cell subtypes in the intestine. COAD database (n = 458) and xCell algorithm was used for the Spearman correlation analysis. The data are representative of three (a, e, f) independent experiments. Error bars represent mean ± SEM. Statistical comparisons were performed using two-tailed unpaired Student’s t test (a-c). n = 5 (a, b-f) independent biological samples. The scale bar represents 100 µm.
